Novel recombinant exosome and use thereof

A method of fusing a membrane fusogenic protein into a cell membrane of a target cell, the method comprising steps of:

• i) preparing an extracellular vesicle comprising a membrane fusogenic protein;
• ii) preparing a target cell to contact with the prepared extracellular vesicle;
• and
• iii) inducing the membrane fusogenic protein of the extracellular vesicle to fuse into a cell membrane of the target cell,
• wherein the fusion in the step (iii) excludes a fusion by endocytosis of the target cell.

A method for delivering a target protein to a target cell in a subject, the method comprising steps of:

• i) preparing an extracellular vesicle comprising a membrane fusogenic protein and a target protein;
• ii) preparing a subject for contacting a target cell of the subject with the prepared extracellular vesicle;
• iii) inducing the membrane fusogenic protein and the target protein of the extracellular vesicle to fuse into a cell membrane of the target cell;
• iv) allowing the target protein to fuse into the cell membrane of the target cell,
• wherein the fusion in the steps (iii) and (iv) excludes a fusion by endocytosis of the target cell.