Optimized cell transplant

An optimized cell transplant, which is characterized by high survival rate and simultaneous high expression of multiple genes, wherein the optimized cell transplant is obtained by the following steps:

• a cell modified culture step:
• mesenchymal stem cells are cultured in a first medium at a cell density of 6,000-15,000 cells/cm2, wherein the surface of a culture dish used has an oxygen-containing functional group and thus features a net negative surface charge due and hydrophilic;
• the first medium is removed after a first culture time to obtain a modified cell intermediate;
• and
• a cell optimized culture step:
• the modified cell intermediate is cultured in a second medium, and the second medium is removed after a second culture time to obtain an optimized cell transplant, wherein
• the mesenchymal stem cells are any one selected from adipose-derived stem cells, bone marrow stem cells, peripheral blood stem cells and umbilical cord blood stem cells;
• the first medium is a serum-free DMEM/F12 medium comprising TGF-b1, FGF-2, hydrocortisone, human serum albumin, L-glutamine, leucine aminopeptidase, an HEPES buffer and lipid;
• the first culture time is between 1 day and 10 days;
• the second culture time is between 7 days and 28 days;
• the optimized cell transplant has simultaneously and highly expressed CD200, Galectin-9 and VISTA genes;
• and
• the expression levels of the CD200, Galectin-9 and VISTA genes of the optimized cell transplant are at least two times higher than those of the mesenchymal stem cells.