Biopesticide composition
OwnerDan LUO, Carina HOLKENBRINK +1inventors
InventorsDan LUO, Carina HOLKENBRINK, Irina BORODINA
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Abstract
The present disclosure relates to a biopesticide composition comprising a target compound selected from (Z,E)-9,11-hexadecadienal, and optionally one or more compounds selected from (Z)-9-hexadecenal, (Z)-11-hexadecenal and/or hexadecanal, in combination with one or more carriers, agents, additives, adjuvants and/or excipients.
Claims61
An E11 fatty acyl-CoA desaturase (E11 desaturase) comprising an amino acid sequence having at least 50% identity to the E11 desaturase comprised in SEQ ID NO:
- 1, SEQ ID NO:
- 80, SEQ ID NO:
- 90, SEQ ID NO:
- 92, SEQ ID NO:
- 96, SEQ ID NO:
- 98, SEQ ID NO:
- 100, SEQ ID NO:
- 102, or SEQ ID NO:
- 104.
A genetically engineered yeast cell producing (Z,E)-9,11-hexadecadienoyl-CoA and (Z,E)-9,11-hexadecadien-1-ol, said cell producing hexadecanoyl-CoA and expressing
- a. a Δ9 desaturase catalyzing the formation of a double bond in a Z configuration in position 9 in the hexadecanoyl-CoA thereby producing a (Z)-9-hexadecenoyl-CoA;
- b. an E11 desaturase catalyzing the formation of a double bond in a E configuration in position 11 in the (Z)-9-hexadecenoyl-CoA thereby producing a (Z,E)-9,11-hexadecadienoyl-CoA;
- and
- c. an alcohol-forming fatty acyl-CoA reductase (FAR) converting the (Z,E)-9,11-hexadecadienoyl-CoA into (Z,E)-9,11-hexadecadien-1-ol.
A method for producing a biopesticide composition comprising the steps:
- (I) culturing a genetically engineered yeast cell producing hexadecanoyl-CoA and expressing
- a. a Δ9 desaturase catalyzing the formation of a double bond in a Z configuration in position 9 in the hexadecanoyl-CoA and thereby producing a (Z)-9-hexadecenoyl-CoA;
- b. an E11 desaturase catalyzing the formation of a double bond in a E configuration in position 11 in the (Z)-9-hexadecenoyl-CoA and thereby producing a (Z,E)-9,11-hexadecadienoyl-CoA;
- c. an alcohol-forming fatty acyl-CoA reductase (FAR) converting the (Z,E)-9,11-hexadecadienoyl-CoA into (Z,E)-9,11-hexadecadien-1-ol;
- (II) enzymatically or chemically converting the (Z,E)-9,11-hexadecadien-1-ol to (Z,E)-9,11-hexadecadienal;
- and
- (III) optionally recovering and/or isolating the (Z,E)-9,11-hexadecadienal and optionally one or more precursors thereof.
A method for producing a biopesticide composition comprising the steps:
- (I) culturing a genetically engineered yeast cell producing hexadecanoyl-CoA and expressing
- a. an E11 desaturase catalyzing the formation of a double bond in a E configuration in position 11 in the hexadecanoyl-CoA and thereby producing an (E)-11-hexadecenoyl-CoA;
- b. a Δ9 desaturase catalyzing the formation of a double bond in a Z configuration in position 9 in the (E)-11-hexadecenoyl-CoA and thereby producing a (Z,E)-9,11-hexadecadienoyl-CoA;
- c. an alcohol-forming fatty acyl-CoA reductase (FAR) converting the (Z,E)-9,11-hexadecadienoyl-CoA into (Z,E)-9,11-hexadecadien-1-ol;
- (II) enzymatically or chemically converting the (Z,E)-9,11-hexadecadien-1-ol to (Z,E)-9,11-hexadecadienal;
- and
- (III) optionally recovering and/or isolating the (Z,E)-9,11-hexadecadienal and optionally one or more precursors thereof.
A biopesticide composition comprising a target compound selected from (Z,E)-9,11-hexadecadienal, and optionally one or more compounds selected from (Z)-9-hexadecenal, (Z)-11-hexadecenal and/or hexadecanal, in combination with one or more carriers, agents, additives, adjuvants and/or excipients.
Dependencies are inferred from the claim text.