US 2026/0253207 A1Published application

Device for processing real-time pcr measurements with a homogeneous reaction medium in which the pcr reagents can diffuse

AssigneeBforcure

InventorsMaël LE BERRE, Francesco VILLA, Stéphane POIRIER

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Abstract

A device includes a memory receiving fluorescence images obtained by filming a real-time PCR reaction in a flat chamber, and an analyzer producing a PCR activity image from the fluorescence images with a homogeneous reaction medium in which PCR reagents can diffuse. The PCR activity image having activity regions in each of which PCR activity is determined as likely based on the fluorescence value of the pixels of these activity regions in the fluorescence images, the pixels of the activity regions each receiving a weighting value. The device further includes an evaluator analyzing the fluorescence images by weighting in each image the fluorescence value of each pixel by the weighting value of that pixel in the activity image, and returning a value indicating whether the filmed real-time PCR reaction exhibits PCR amplification, a threshold cycle value, and a concentration curve during the PCR reaction.

Claims16

Dependent claims

A device for processing real-time PCR measurements with a homogeneous reaction medium in which the PCR reagents can diffuse, the device comprising:

a memory configured to receive a plurality of fluorescence images obtained by filming a real-time PCR reaction in a flat chamber,
an analyzer configured to produce a PCR activity image from the plurality of fluorescence images with a homogeneous reaction medium in which the PCR reagents can diffuse, which PCR activity image has a plurality of activity regions in each of which PCR activity is determined as likely on the basis of the fluorescence value of the pixels of these activity regions in the plurality of fluorescence images, each of the pixels of the activity regions receiving a weighting value associated with the likelihood of a PCR reaction such that the sum of all weighting values is one, and the pixels outside the activity regions receiving a zero value,
an evaluator configured to analyze the plurality of fluorescence images obtained by filming a real-time PCR reaction in a flat chamber by weighting in each image the fluorescence value of each pixel by the weighting value of that pixel in the activity image, and to return one or more among a value indicating whether the filmed real-time PCR reaction exhibits PCR amplification, a threshold cycle value, and a concentration curve during the PCR reaction.

A method for processing real-time PCR measurements with a homogeneous reaction medium in which the PCR reagents can diffuse, the method comprising:

a) receiving a plurality of fluorescence images obtained by filming a real-time PCR reaction in a flat chamber with a homogeneous reaction medium in which the PCR reagents can diffuse,
b) producing a PCR activity image from the plurality of fluorescence images, which PCR activity image has a plurality of activity regions in each of which PCR activity is determined to be likely on the basis of the fluorescence value of the pixels of these activity regions in the plurality of fluorescence images, each of the pixels of the activity regions receiving a weighting value associated with the likelihood of a PCR reaction such that the sum of all values is one, and the pixels outside the activity regions receiving a zero value,
c) analyzing the plurality of fluorescence images by weighting in each image the fluorescence value of each pixel by the weighting value of that pixel in the activity image, and to return one or more among a value indicating whether the filmed real-time PCR reaction exhibits PCR amplification, a threshold cycle value, and a concentration curve during the PCR reaction.

Dependencies are inferred from the claim text.