Methods for distinguishing lung cancer from non-cancer

A method for detecting lung cancer associated proteomic markers, the method comprising:

• (a) creating a dataset comprising a plurality of lung cancer associated proteomic markers from lung cancer samples and non-cancer samples, wherein the lung cancer samples are no more than 20% of samples used to create the dataset;
• (b) training a classifier with the dataset in (a) to generate a trained classifier, wherein the trained classifier distinguishes lung cancer samples from non-cancer samples;
• (c) obtaining a biofluid sample from a subject;
• (d) measuring an amount or a concentration of the lung cancer associated proteomic markers in the biofluid sample or a processed sample therefrom to obtain proteomic measurements;
• and
• (e) applying the trained classifier to the proteomic measurements to provide a quantitative or qualitative result for the biofluid sample of the lung cancer.