Optical nanosensors for hydrolytic enzyme characterization
AssigneeIowa State University Research Foundation, Inc.
InventorsNigel Forest Reuel, Nathaniel Kallmyer
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Abstract
Various embodiments disclosed relate to a sensor assembly probe for determining enzymatic activity. The sensor assembly probe includes an aqueous medium including one or more fluorescent hydrophobic semi-conductive nanoparticles dispersed therein. The assembly further includes an ionic polymer coating at least a portion of a surface of the one or more fluorescent hydrophobic semi-conductive nanoparticles. The assembly further includes a substrate for an enzyme, the substrate disposed between at least two of the one or more fluorescent hydrophobic semi-conductive nanoparticles, the substrate including at least ionic group.
Claims23
A sensor assembly probe for determining enzymatic activity comprising:
- an aqueous medium comprising one or more fluorescent hydrophobic semi-conductive nanoparticles dispersed therein;
- an ionic polymer coating at least a portion of a surface of the one or more fluorescent hydrophobic semi-conductive nanoparticles;
- a substrate for an enzyme, the substrate disposed between at least two of the one or more fluorescent hydrophobic semi-conductive nanoparticles, the substrate comprising at least ionic group.
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Dependencies are inferred from the claim text.