System for label-free electrochemical sensing

Abstract

The disclosure relates generally to electrodes with a target-binding molecule conjugated on its surface that is overlaid with a polymer of sufficient thickness to restrict ion mobility so that the electrical double layer is moved closer to the site of target binding to the immobilized target-binding molecule. Generally, thickness of the polymer coating layer is such that a target-binding site of the immobilized target-binding molecule is not in the coating layer, e.g., the target-binding site is exposed. The disclosure also provides sensors comprising the electrode and uses of the electrodes and sensors in target analyte detection, e.g., in label-free detection of targets. WO

Dependencies are inferred from the claim text.