Assay of rna poly(a) tail length
AssigneeCellscript, LLC
InventorsJerome J. Jendrisak, Ronald J. Meis, Kari Weber, Gary A. Dahl
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Abstract
The present invention relates to compositions and kits and to methods, and uses of any thereof to accurately and precisely assay the size in terms of number of adenosines and the size distribution of polyadenylic acid (“polyA” or “poly(A)” tails) on the 3′-end portions of RNA molecules.
Claims21
A polyA size marker ladder comprising a collection of single-stranded polyadenylic acid oligoribonucleotides (polyA oligos) of different lengths, each having a 5′-monophosphate and 3′-hydroxy group, wherein, the length in terms of the number of adenosines in the smallest of said polyA oligos in said collection is a first integer X selected from the group consisting of all integers from 10 to 30, and each next larger polyA oligo in said collection increases in number of adenosines by the same first integer X, and
- wherein the total number of adenosines in the largest poly(A) oligo in said collection is the first integer X multiplied by a second integer Y, wherein the second integer Y is the total number of different lengths of polyA oligos composing said polyA size marker ladder.
The polyA size marker ladder of, wherein:
- A. said polyA size marker ladder further comprises at least one reference tag oligo comprising or consisting of a single-stranded DNA (ssDNA) oligo, and/or
- B. said polyA size marker ladder further comprises at least reference tag oligo comprising a single-stranded RNA (ssRNA) oligo;
- wherein said reference tag oligo in A and/or B has at least one feature that enables it to be a reference tag oligo for identifying the size of at least one polyA oligo in said polyA size marker ladder based on where said at least one polyA oligo migrates during electrophoretic separation compared to said at least one reference tag oligo.
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Dependencies are inferred from the claim text.