Systems and methods for detecting pesticide resistance
AssigneeClarke Mosquito Control Products, Inc.
InventorsMariana Lizbeth Jiménez Martinez, Iram Pablo Rodríguez Sánchez, Gerardo de Jesús Trujillo Rodríguez, Everarado González González
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Abstract
Methods and detection kits for identification of pesticide resistance in pests. The kits may be suitable for the detection of resistance in the field as they allow a quick and simple identification of a resistance marker with a small sample size. Further disclosed are methods for resistance management in pests that are resistant to certain pesticides.
Claims13
A kit for determining resistance of a pest to a pesticide, the kit comprising:
- a system for maceration of the pest;
- a primer solution comprising one or more primer pairs, wherein each primer pair binds to a specific microRNA (miRNA);
- and,
- a colorimetric system for detecting the presence or absence of the specific miRNA.
A method of detecting resistance of pests to a pesticide, the method comprising:
- collecting a sample of pests;
- macerating the sample of pests;
- incubating a primer solution with the macerated sample of pests, the primer solution comprising one or more primer pairs, wherein each primer pair binds to a specific microRNA (miRNA);
- and
- performing a colorimetric-based detection of the miRNA.
A method of controlling one or more pesticide resistant pests, the method comprising:
- collecting a sample of pests;
- macerating the sample of pests;
- incubating a primer solution with the macerated sample of pests, the primer solution comprising one or more primer pairs, wherein each primer pair binds to a specific microRNA (miRNA) and the specific miRNA corresponds to a specific pesticide;
- performing a colorimetric-based detection of the specific miRNA;
- when the specific miRNA is detected, administering to the pests a pesticide different than the specific pesticide.
Dependencies are inferred from the claim text.