Method of dna synthesis

An in vitro cell-free process for production of a deoxyribonucleic acid (DNA) molecule which comprises a desired DNA sequence, the process comprising:

• (a) contacting an oligonucleotide immobilised on a solid support with a series of template oligonucleotides which overlap in sequence, in a presence of at least one DNA polymerase, under conditions promoting template-dependent extension of the immobilised oligonucleotide to produce a first DNA strand which comprises the desired DNA sequence and further comprises a first portion of a protelomerase target sequence proximal to the solid support;
• (b) using the first DNA strand as a template for extension of a second DNA strand complementary to the first DNA strand and introducing a DNA sequence comprising a second portion of the protelomerase target sequence of (a) on the second DNA strand, such that the first portion and the second portion of the protelomerase target sequence thereby create a complete protelomerase target sequence proximal to the solid support;
• and
• (c) contacting the protelomerase target sequence proximal to the solid support with a protelomerase, under conditions promoting cleavage and rejoining of the protelomerase target sequence, to thereby release the produced DNA molecule from immobilisation.