Method of producing negative-strand rna virus vector and produced negative-strand rna virus vector
AssigneeRepli-Tech Co., Ltd.
InventorKoichi SAEKI
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Abstract
The present disclosure provides a method of producing a negative-strand RNA virus vector. The present disclosure specifically provides a method of producing a negative-strand RNA virus vector in the presence of a PKR inhibitory factor.
Claims16
A method of producing a negative-strand RNA virus or virus vector, the method comprising:
- expressing a protein kinase R (PKR) inhibitory factor from a gene encoding the PKR inhibitory factor operably linked to a regulatory sequence and supplying it to a packaging cell;
- causing the packaging cell to express a genomic RNA of the negative-strand RNA virus or virus vector to form the negative-strand RNA virus or virus vector in the presence of the PKR inhibitory factor;
- and
- recovering the formed negative-strand RNA virus or virus vector;
- wherein:
- the PKR inhibitory factor is a PKR inhibitory viral factor, or nc886 or p58IPK;
- and
- the relationship between the virus or virus vector and the PKR inhibitory factor is heterologous, and/or the relationship between the regulatory sequence and the PKR inhibitory factor is heterologous.
An RNA genome of a negative-strand RNA virus or virus vector, comprising expressibly a gene encoding any one or more of PKR inhibitory factors.
A packaging cell for a negative-strand RNA virus or viral vector, having a gene encoding a protein kinase R (PKR) inhibitory factor operably linked to a regulatory sequence.
Use of a packaging cell having a gene encoding a protein kinase R (PKR) inhibitory factor operably linked to a regulatory sequence in the production of a negative-strand RNA virus or viral vector.
Dependencies are inferred from the claim text.