Methods for assessing resistance to non-integrating virus vectors

An in vitro method for assessing the ability of a subject to produce at least one therapeutic molecule of interest encoded by a non-integrative viral vector, comprising the steps of:

• (i) culturing cells expressing an attachment receptor specific of said non-integrating viral vector;
• (ii) incubating said cells with a composition comprising said non-integrating viral vector and a serum sample of said subject in a culture medium;
• (iii) measuring the expression level of said at least one therapeutic molecule of interest encoded by said non-integrative viral vector;
• (iv) comparing the expression level measured in step (iii) with a reference value;
• (v) assessing whether the subject is able to produce the at least one molecule of interest, wherein an expression level of the at least one molecule of interest superior to a reference value means that the subject is able to produce the at least one molecule of interest.

An in vitro method to assess the susceptibility of a subject to respond to a treatment with a non-integrative viral vector encoding at least one molecule of interest, comprising the steps of:

• (i) culturing cells expressing an attachment receptor specific of the non-integrating viral vector;
• (ii) incubating the cells with a composition comprising the non-integrating viral vector and a serum sample of the subject in a culture medium;
• (iii) measuring the expression level of the at least one molecule of interest;
• and
• (iv) assessing if the subject is susceptible to respond to the treatment, wherein an expression level of the at least one molecule of interest superior to a reference value means that the subject is susceptible to respond to the treatment.

A method for preventing and/or treating a disease with a non-integrative viral vector encoding at least one molecule of interest in a subject, comprising the steps of:

• determining if the subject is susceptible to respond to a treatment with the non-integrative viral vector encoding the at least one molecule of interest by:
• (i) culturing cells expressing an attachment receptor specific of the non-integrating viral vector;
• (ii) incubating the cells with a composition comprising the non-integrating viral vector encoding the at least one molecule of interest, and a serum sample of the subject in a culture medium;
• (iii) measuring the expression level of the at least one molecule of interest;
• and
• (iv) determining if the subject is susceptible to respond to a treatment with the non-integrative viral vector, wherein an expression level of the at least one molecule of interest superior to a reference value means that the subject is susceptible to respond, and
• administering to the subject susceptible to respond a therapeutically effective amount of the non-integrative viral vector encoding at least one molecule of interest.