Asian soybean rust resistance genes

A soybean plant, plant cell, or seed comprising stably incorporated in its genome a heterologous polynucleotide comprising a nucleotide sequence selected from the group consisting of:

• (a) the nucleotide sequence set forth in SEQ ID NO:
• 1, 2, 4, 5, 7, 9, 10, 11, 13, 14, 16, 18, 19, 20, 22, 23, 25, 27, 28, or 32;
• (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
• 6, 8, 15, 17, 24, or 26;
• (c) a nucleotide sequence having at least 80% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 2, 5, 11, 14, 20, 23, 27, and 32, wherein the nucleic acid molecule is capable of conferring resistance to Asian soybean rust (ASR) caused by at least one race of Phakopsora pachyrhizi, relative to a control plant not comprising the nucleic acid molecule;
• (d) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 6, 15, and 24, wherein the nucleic acid molecule is capable of conferring resistance to ASR caused by at least one race of P. pachyrhizi, relative to a control plant not comprising the nucleic acid molecule;
• (e) a nucleotide sequence having at least 80% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 4, 7, 13, 16, 22, 25, and 28, wherein the nucleic acid molecule is capable of modulating in a plant the activity of at least one R gene therein, relative to a control plant not comprising the nucleic acid molecule;
• and
• (f) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 8, 17, and 26, wherein the nucleic acid molecule is capable of modulating in a plant the activity of at least one R gene therein, relative to a control plant not comprising the nucleic acid molecule.

A method for enhancing the resistance of a soybean plant to ASR caused by at least one race of P. pachyrhizi, the method comprising introducing into at least one soybean plant cell a heterologous polynucleotide and regenerating a soybean plant comprising in its genome the heterologous polynucleotide, wherein the heterologous polynucleotide comprising a nucleotide sequence selected from the group consisting of:

• (a) the nucleotide sequence set forth in SEQ ID NO:
• 1, 2, 5, 9, 10, 11, 14, 18, 19, 20, 23, 27, or 32;
• (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
• 6, 15, and 24;
• (c) a nucleotide sequence having at least 80% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 2, 5, 11, 14, 20, 23, 27, and 32, wherein the nucleic acid molecule is capable of conferring resistance to Asian soybean rust (ASR) caused by at least one race of Phakopsora pachyrhizi, relative to a control plant not comprising the nucleic acid molecule;
• and
• (d) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 6, 15, and 24, wherein the nucleic acid molecule is capable of conferring resistance to ASR caused by at least one race of P. pachyrhizi, relative to a control plant not comprising the nucleic acid molecule.

A method for identifying a plant that comprises an R gene and/or a modulator gene, the method comprising detecting in the plant, or in at least one part or cell thereof, the presence of a nucleotide sequence selected from the group consisting of:

• the nucleotide sequence set forth in SEQ ID NO:
• 1, 2, 4, 5, 7, 9, 10, 11, (a) 13, 14, 16, 18, 19, 20, 22, 23, 25, 27, 28, or 32;
• (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
• 6, 8, 15, 17, 24, or 26;
• (c) a nucleotide sequence having at least 80% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 2, 5, 11, 14, 20, 23, 27, and 32, wherein the nucleic acid molecule is capable of conferring resistance to Asian soybean rust (ASR) caused by at least one race of Phakopsora pachyrhizi, relative to a control plant not comprising the nucleic acid molecule;
• (d) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 6, 15, and 24, wherein the nucleic acid molecule is capable of conferring resistance to ASR caused by at least one race of P. pachyrhizi, relative to a control plant not comprising the nucleic acid molecule;
• (e) a nucleotide sequence having at least 80% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 4, 7, 13, 16, 22, 25, and 28, wherein the nucleic acid molecule is capable of modulating in a plant the activity of at least one R gene therein, relative to a control plant not comprising the nucleic acid molecule;
• and
• (f) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 8, 17, and 26, wherein the nucleic acid molecule is capable of modulating in a plant the activity of at least one R gene therein, relative to a control plant not comprising the nucleic acid molecule.

A polypeptide comprising an amino acid sequence selected from the group consisting of:

• (a) the amino acid sequence set forth in SEQ ID NO:
• 6, 15, and 24;
• (b) the amino acid sequence set forth in SEQ ID NO:
• 8, 17, and 26;
• (c) an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 6, 15, and 24, wherein a polypeptide comprising the amino acid sequence is capable of conferring resistance to ASR caused by at least one race of P. pachyrhizi, relative to a control plant not comprising the polypeptide;
• and
• (d) an amino acid sequence having at least 80% sequence identity to at least one of the amino acid sequences set forth in SEQ ID NO:
• 8, 17, and 26, wherein a polypeptide comprising the amino acid sequence is capable of modulating in a plant the activity of at least one R gene therein, relative to a control plant not comprising the polypeptide.

A promoter comprising a nucleotide sequence selected from the group consisting of:

• (a) the nucleotide sequence set forth in SEQ ID NO:
• 3, 12, 21, 29, 30, and 31;
• and
• (b) a nucleotide sequence having at least 90% sequence identity to at least one of the nucleotide sequences set forth in SEQ ID NOS:
• 3, 12, 21, 29, 30, and 31, wherein the promoter is capable of driving the expression on an operably linked polynucleotide in a plant cell.