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US 2026/0250677 A1Published application
Catalytic nucleic acid-based genetic engineering method
AssigneesThe Board of Trustees of the University of Illinois, Board of Regents, The University of Texas System
InventorsYi Lu, Mingkuan Lyu, Linggen Kong
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Abstract
Systems and kits are disclosed herein for genetic engineering (such as for DNA cleavage and gene-editing), which include catalytic nucleic acids and catalytic nucleic acid-assisting reagents. Methods of genetic engineering are also described, in which both catalytic nucleic acid-assisting reagents and catalytic nucleic acids are specific for a target site, thus, providing high-fidelity genetic engineering.
Claims20
A system for genetic engineering, comprising:
- one or more catalytic nucleic acids, comprising:
- one or more catalytic domains;
- and
- one or more binding domains, wherein the one or more binding domains comprise a sequence complementary to and/or identical to a sequence flanking a target site and/or within the target site;
- and
- one or more catalytic nucleic acid-assisting reagents, comprising a sequence that specifically binds a sequence that is complementary to and/or identical to a sequence flanking the target site and/or within the target site.
3 more elements
A method of genetic engineering, comprising:
- contacting a target nucleic acid with one or more catalytic nucleic acid-assisting reagents, wherein:
- the one or more catalytic nucleic acid-assisting reagents comprise a sequence that specifically binds a sequence that is complementary to and/or identical to a sequence flanking a target site and/or within a target site;
- and
- the contacting occurs under conditions sufficient for specific binding between the target nucleic acid and the one or more catalytic nucleic acid-assisting reagents, generating a catalytic nucleic acid-assisting reagent-bound target nucleic acid;
- and
- contacting the catalytic nucleic acid-assisting reagent-bound target nucleic acid with one or more catalytic nucleic acids;
- wherein:
- the one or more catalytic nucleic acids comprise:
- one or more catalytic domains;
- and
- one or more binding domains, wherein the one or more binding domains comprise a sequence complementary to a sequence flanking the target site;
- and
- the contacting occurs under conditions sufficient for catalytic nucleic acid activity.
10 more elements
Dependencies are inferred from the claim text.