Bio-fluid's ligand library enrichment technology and uses thereof
AssigneeEisai R&D Management Co., Ltd.
InventorYogo Sakakibara
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Abstract
The present disclosure, at least in part, provides methods for generating aptamer libraries capable from binding to multiple target molecules (e.g., one or more biomolecules) in a complexed sample (e.g., biological sample) using electrophoresis mobility shift (e.g., 2-dimensional electrophoresis). The present disclosure also provides databases comprising information from one or more aptamer library (e.g., aptamer library generated by the methods described herein) and methods of utilizing such aptamer libraries (e.g., by comparing the information of a reference aptamer library and a target aptamer library to discern the differences).
Claims27
A method for enriching a plurality of aptamers capable of binding to one or more biomolecules in a biological sample, the method comprising:
- (i) contacting a plurality of candidate aptamers with a biological sample to form a composition comprising a plurality of aptamer-biomolecule complexes;
- (ii) subjecting the composition to electrophoresis in a first electrophoresis medium in a first direction to obtain a portion of the first electrophoresis medium that comprises the plurality of aptamer-biomolecule complexes;
- (iii) subjecting the portion of the first electrophoresis medium to electrophoresis in a second electrophoresis medium in a second direction to obtain a portion of the second electrophoresis medium that comprises the plurality of aptamer-biomolecule complexes;
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- (iv) extracting the plurality of aptamers capable of binding to biomolecules in the biological sample from the plurality of aptamer-biomolecule complexes.
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Dependencies are inferred from the claim text.