US 2026/0250658 A1 · Lysis/Binding Solution, Cleaning Solution, Kit and Method for Nucleic Acid Extraction

Abstract

A lysis/binding solution for nucleic acid extraction is provided. The lysis/binding solution for nucleic acid extraction includes a protein denaturant, a non-alcohol dehydrating agent, a first surfactant, a buffer solution, ethylenediaminetetraacetic acid, and a chaotropic salt. A molarity of the protein denaturant ranges from 3.5 mol/L to 5.5 mol/L, a weight by volume percent of the non-alcohol dehydrating agent ranges from 0.1% to 1%, a weight by volume percent of the first surfactant ranges from 0.05% to 0.5%, a molarity of the buffer solution ranges from 5 mmol/L to 150 mmol/L, a molarity of the ethylenediaminetetraacetic acid ranges from 5 mmol/L to 20 mmol/L, and a weight by volume percent of the chaotropic salt ranges from 1% to 8%.

Claims23

Dependent claims

A lysis/binding solution for nucleic acid extraction, the lysis/binding solution comprising:

a protein denaturant, a non-alcohol dehydrating agent, a first surfactant, a buffer solution, ethylenediaminetetraacetic acid, and a chaotropic salt, wherein
a molarity of the protein denaturant ranges from 3.5 mol/L to 5.5 mol/L, a weight by volume percent of the non-alcohol dehydrating agent ranges from 0.1% to 1%, a weight by volume percent of the first surfactant ranges from 0.05% to 0.5%, a molarity of the buffer solution ranges from 5 mmol/L to 150 mmol/L, a molarity of the ethylenediaminetetraacetic acid ranges from 5 mmol/L to 20 mmol/L, and a weight by volume percent of the chaotropic salt ranges from 1% to 8%.

A method for nucleic acid extraction, the method comprising:

adding a lysis/binding solution and a proteinase K solution to a sample to be performed with nucleic acid extraction and mixing the lysis/binding solution, the proteinase K solution and the sample to form a mixed solution, at a temperature condition of 55° C. to 80° C.;
adding a magnetic bead suspension to the mixed solution, mixing the magnetic bead suspension and the mixed solution, placing the magnetic bead suspension and the mixed solution on a magnetic rack for standing, and removing a supernatant to obtain a second precipitate;
adding a first cleaning solution to the second precipitate, mixing the first cleaning solution and the second precipitate, placing the first cleaning solution and the second precipitate on a magnetic rack for standing, and removing a supernatant, to obtain a third precipitate;
adding a second cleaning solution to the third precipitate, mixing the second cleaning solution and the third precipitate, placing the second cleaning solution and the third precipitate on a magnetic rack for standing, and removing a supernatant to obtain a fourth precipitate;
and
adding an eluent solution to the fourth precipitate and mixing the eluent solution and the fourth precipitate, at a temperature condition of 40° C. to 60° C., placing the eluent solution and the fourth precipitate on a magnetic rack for standing, and removing a supernatant to obtain extracted nucleic acids.

3 more elements

Dependencies are inferred from the claim text.

Lysis/Binding Solution, Cleaning Solution, Kit and Method for Nucleic Acid Extraction

Life of this patent

Lysis/Binding Solution, Cleaning Solution, Kit and Method for Nucleic Acid Extraction

Life of this patent

Abstract

Claims23