Method for eliminating carryover contamination in nucleic acid amplification reaction using uracil-dna glycosylase derived from photobacterium leiognathi

Abstract

Novel uracil-DNA glycosylase (UDG) derived from Photobacterium leiognathi and uses thereof are disclosed. The novel UDG is an enzyme isolated from Photobacterium leiognathi, which is a psychrophilic bacterium, and is characterized by having an activity of degrading uracil bases from uracil-containing DNA substrate and being easily denatured at low temperatures. Accordingly, the UDG can be effectively used to eliminate carryover contamination occurring during the nucleic acid amplification reaction, resulting in increased accuracy (elimination of false positives), purity and amplification efficiency in nucleic acid amplification reactions.

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