Methods for overcoming glutamine deprivation during mammalian cell culture
AssigneesBiogen MA Inc., Samsung Bioepis Co., Ltd.
InventorsWilliam C. Yang, Yao-Ming Huang, Marty Sinacore, Thomas Ryll, Jiuyi Lu, Jaesun Lee, Kyngju Lee, Hyoung Taek Lim, Eunchong Yang
Life of this patent
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Abstract
The present invention pertains to methods of producing polypeptide of interest in cell cultures lacking glutamine. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with nucleic acid synthesis precursors and/or corticosteroids.
Claims16
A method of producing a polypeptide of interest in a glutamine-free cell culture media, comprising culturing mammalian cells expressing the polypeptide of interest in a cell culture medium under conditions that support expression of the polypeptide of interest, wherein said cell culture medium comprises between about 20 μM and about 1000 μM hypoxanthine and between about 2 μM and about 1000 μM thymidine.
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(canceled)
A method of producing a polypeptide of interest in a glutamine-free cell culture media, comprising culturing mammalian cells expressing the polypeptide of interest in a cell culture medium under conditions that support expression of the polypeptide of interest, wherein said cell culture medium comprises between about 0.5 μM and about 500 μM of a corticosteroid.
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Dependencies are inferred from the claim text.