Chimeric nanobody-decorated liposomes by self-assembly
AssigneeThe Research Foundation for The State University of New York
InventorsYuan Wan, Mofizur Rahman
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Abstract
An engineered nanobody conjugate is provided, comprising: a Variable Heavy domain of Heavy chain (VHH) nanobody; a peptide single transmembrane domain (STMD); and a peptide linker between the nanobody and the STMD. The invention also provides a method of making an immunoliposome composition, comprising: mixing the engineered nanobody according to claim 1 with a liposome composition; and forming 100 nm liposomes from the liposome composition, having between 200 to 2500 chimeric nanobodies per 100 nm liposome. An engineered nanobody conjugate is provided comprising an ordered sequence of a peptide affinity tag, a Variable Heavy domain of Heavy chain (VHH) nanobody, a peptide linker, a cysteine residue, and a peptide fatty acylation signal.
Claims20
An engineered nanobody conjugate, comprising:
- a nanobody;
- a peptide transmembrane domain or a peptide fatty acylation signal;
- and
- a peptide linker between the nanobody and the peptide transmembrane domain.
An engineered nanobody conjugate comprising an ordered sequence of:
- a peptide affinity tag,
- a Variable Heavy domain of Heavy chain (VHH) nanobody,
- a peptide linker,
- a cysteine residue, and
- a peptide fatty acylation signal.
Dependencies are inferred from the claim text.