Function-enhanced engineered ebna1 for protein expression in mammalian cells
AssigneeWuxi Biologics Ireland Limited
InventorsShanhui Liao, Huihui Lu, Qian Guo, Xiaoyue Chen, Jiexing Cai, Weichang Zhou
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Abstract
Provided herein are engineered Epstein-Barr virus nuclear antigen 1 (EBNA1), coding molecules thereof, vectors and mammalian cell expression systems comprising the same, and polypeptide of interest recombinantly produced by the foregoing. Also provided are methods for the preparation of the engineered EBNA1s, coding molecules thereof, vectors and mammalian cell expression systems and methods for using the same in recombinant expression.
Claims30
An engineered Epstein-Barr virus nuclear antigen 1 (EBNA1), wherein as compared to wild-type EBNA1, the original G-A rich region is engineered in the engineered EBNA1.
An isolated mammalian cell suitable for recombinant expression of a polypeptide of interest, wherein the mammalian cell comprises an engineered EBNA1, wherein the engineered EBNA1 has enhanced function in recombinant expression as compared to wild-type EBNA1 (such as a wild-type EBNA1 comprises an amino acid sequence of SEQ ID NO:
- 1) in improving the recombinant expression, and wherein the original G-A rich region of wild-type EBNA1 is engineered in the engineered EBNA1.
Dependencies are inferred from the claim text.