Mastermix integration into cartridge-based in vitro diagnostics device

A method of forming a cartridge to be used for nucleic acid amplification, the method comprising:

• dispensing a first droplet of a first solution into a first well arranged along a flow path of the cartridge, wherein the first solution contains a first subgroup of Mastermix reagents, and wherein the first droplet makes contact with walls of the first well when the first droplet is received within the first well;
• dispensing a second droplet of a second solution into a second well arranged along the flow path of the cartridge, wherein the second solution contains a second subgroup of Mastermix reagents, and wherein the second droplet makes contact with walls of the second well when the second droplet is received within the second well;
• dispensing a third droplet of a third solution into a third well arranged along the flow path of the cartridge, wherein the third solution contains a third subgroup of Mastermix reagents, and wherein the third droplet makes contact with walls of the third well when the third droplet is received within the third well;
• and
• drying the first droplet, the second droplet, and the third droplet,
• wherein, collectively, the Mastermix reagents in the first subgroup, the second subgroup, and the third subgroup comprise all reagents needed for performing nucleic acid amplification.