Topographic modulation of enzymatic reaction affords ultrasensitive compartment-free digital immunoassays
OwnerYong ZENG, Yunjie WENinventors
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Abstract
In one aspect, the disclosure relates to a system comprising a plurality of microposts protruding from a membrane opposite a solid substrate, wherein the system can be pneumatically actuated to bring the microposts closer to the solid substrate. Also disclosed herein are methods of constructing the system, methods of performing digital bioassays using the disclosed system, and noninvasive methods for detecting diseases and for monitoring the progress of disease treatments using the disclosed system. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
Claims22
A system comprising:
- (a) a first layer comprising a solid substrate;
- (b) a second layer comprising a plurality of microposts;
- (c) a third layer;
- and
- (d) a pneumatic actuator;
- wherein the plurality of microposts protrude towards the first layer;
- wherein the second layer is separated by an air gap from the third layer;
- and
- wherein the pneumatic actuator can be activated to create a nanogap between the first layer and the plurality of microposts and to increase or decrease a height of the nanogap between the first layer and the plurality of microposts by increasing or decreasing a height of the air gap, thereby changing a position of the second layer.
-50. (canceled)
Dependencies are inferred from the claim text.