Minoxidil-crosslinked injectable hydrogels for local and sustained intradermal deliver y

A method for producing a sustained delivery hydrogel comprising a crosslinked minoxidil-aldehyde-modified polymer, the method comprising:

• (a) preparing an aldehyde-modified polymer;
• (b) dissolving the aldehyde-modified polymer in a minoxidil solution comprising phosphate buffered saline;
• and
• (c) mixing the minoxidil solution comprising the dissolved aldehyde-modified polymer to react aldehydes of the aldehyde-modified polymer and primary amines of the minoxidil, thereby forming the hydrogel.

A method for producing a sustained delivery hydrogel comprising a crosslinked minoxidil-aldehyde-modified hyaluronic acid derivative, the method comprising:

• (a) preparing an aldehyde-modified hyaluronic acid derivative;
• (b) dissolving the aldehyde-modified hyaluronic acid derivative in a minoxidil solution comprising phosphate buffered saline;
• and
• (c) mixing the minoxidil solution comprising the dissolved aldehyde-modified polymer to react aldehydes of the aldehyde-modified polymer and primary amines of the minoxidil, thereby forming the hydrogel.

A method for treating hair loss in a subject in need thereof, the method comprising administering a sustained delivery hydrogel comprising a crosslinked minoxidil-aldehyde-modified polymer to an area of hair loss of the subject,

• wherein administration comprises:
• (a) transdermal administration comprising subcutaneous injection, microneedle array injection or application of a transdermal patch comprising the hydrogel;
• (b) local administration comprising topical application of an ointment, gel, nanoparticles or liposomes comprising the hydrogel;
• or
• (c) a combination of (i) skin needling, and (ii) the local administration of (b).

A method for treating hair loss in a subject in need thereof, the method comprising administering a sustained delivery hydrogel comprising a crosslinked

• minoxidil-aldehyde-modified hyaluronic acid derivative to an area of hair loss of the subject,
• wherein administration comprises:
• (c) transdermal administration comprising subcutaneous injection, microneedle array injection or application of a transdermal patch comprising the hydrogel;
• (d) local administration comprising topical application of an ointment, gel, nanoparticles or liposomes comprising the hydrogel;
• or
• (c) a combination of (i) skin needling, and (ii) the local administration of (b).

A method for prolonging anagen in a subject having hair loss, the method comprising administering a sustained delivery hydrogel comprising a crosslinked

• minoxidil-aldehyde-modified hyaluronic acid derivative to an area of hair loss of the subject,
• wherein administration comprises:
• (a) transdermal administration comprising subcutaneous injection, microneedle array injection or application of a transdermal patch comprising the hydrogel;
• (b) local administration comprising topical application of an ointment, gel, nanoparticles or liposomes comprising the hydrogel;
• or
• (c) a combination of (i) skin needling, and (ii) the local administration of (b).

A method for prolonging anagen in a subject having hair loss, the method comprising administering a sustained delivery hydrogel comprising a crosslinked minoxidil-aldehyde-modified polymer to an area of hair loss of the subject,

• wherein administration comprises:
• (a) transdermal administration comprising subcutaneous injection, microneedle array injection or application of a transdermal patch comprising the hydrogel;
• (b) local administration comprising topical application of an ointment, gel, nanoparticles or liposomes comprising the hydrogel;
• or
• (c) a combination of (i) skin needling, and (ii) the local administration of (b).