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AssigneesBeijing Forestry University, Jilin Allgenes Science &Technology Co., Ltd
InventorsZhengrong Yuan, Fengcheng Song, Xichun Li, Guopeng Liang, Biao Ma, Wei Wang, Yiran Cheng, Shuaishuai Feng, Lei Yu, Jiaxin Cui
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The disclosure belongs to the technical field of animal bioengineering. Disclosed is a method for knocking out a bovine FBXO40 gene and an application thereof. A CRISPR/Cas12i system is used to knock out the bovine FBXO40 gene. The CRISPR/Cas12i system targets exon 5 of the bovine FBXO40 gene, and a nucleotide sequence of the exon 5 of the bovine FBXO40 gene is shown in SEQ ID No. 1. In the disclosure, a method for precise knockout of the bovine FBXO40 gene using a CRISPR/Cas12i gene editing technology is provided, and stably inheritable monoclonal cell lines and embryos with gene knockouts are successfully constructed. The method can provide experimental references for studying a molecular mechanism for bovine skeletal muscle development, and can also be applied in the breeding of new cattle breeds with high-meat-yielding.
A method for knocking out a bovine FBXO40 gene, using a CRISPR/Cas12i system to knock out the bovine FBXO40 gene, wherein the CRISPR/Cas12i system targets exon 5 of the bovine FBXO40 gene, a nucleotide sequence of the exon 5 of the bovine FBXO40 gene being as shown in SEQ ID No. 1, and the CRISPR/Cas12i system targets nucleotides at position 178 to position 197 from a 5′ terminus in the sequence of SEQ ID No. 1.
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