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AssigneePioneer Hi-Bred International, Inc.
InventorsSREEKALA CHELLAMMA, NATHAN ALLEN DAVIS, TARA LUANA BARRETT TARNOWSKI, MARIA LAURA MAYOR, REBEKAH ANNE COURTER-CRISPIN, STEPHEN MICHAEL SCHRADER
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Three sorghum maternal haploid inducer lines 2PWCS25R, 2PNUM88R, and 2PCNW79R are provided including seed, plants and plant parts thereof. Methods for producing sorghum haploid embryos using any of 2PWCS25R, 2PNUM88R, or 2PCNW79R are also provided. The sorghum haploid embryos/seeds/plants produced as a result of the use of any of maternal haploid inducer line 2PWCS25R, 2PNUM88R, or 2PCNW79R may be doubled to produce doubled haploid embryos, seeds, and plants (e.g., as part of a sorghum breeding program). Also provided is a method of generating a haploid sorghum embryo comprising performing emasculation on a first sorghum plant. The emasculation comprises contacting the first plant with Trifluoromethanesulfonamide (TFMSA).
A plant, non-seed plant part, seed, or cell of sorghum variety 2PWCS25R, sorghum variety 2PNUM88R, or sorghum variety 2PCNW79R, representative seed of the variety having been deposited with the Nation Center for Marine Algae and Microbiota (NCMA) under accession number 202407001, 202407002, or 202407003, respectively.
A method of producing a sorghum haploid embryo or seed, the method comprising pollinating a female sorghum diploid plant with pollen from sorghum haploid inducer line 2PWCS25R, sorghum haploid inducer line 2PNUM88R, or sorghum haploid inducer line 2PCNW79R, representative seed of the line having been deposited with the Nation Center for Marine Algae and Microbiota (NCMA) under accession number 202407001, 202407002, or 202407003, respectively, wherein the pollination results in production of a sorghum haploid embryo and/or haploid seed.
A method of generating a haploid sorghum embryo comprising
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