Novel disease resistant watermelon plants
AssigneeSyngenta Crop Protection AG
InventorsMatthew KINKADE, Carine RIZZOLATTI, Marc OLIVER, Kishor BHATTARAI, Ajay SANDHU
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Abstract
The present invention relates to novel watermelon plants displaying an increased resistance to Fusarium oxysporum f.sp. niveum race 2 infection. The present invention also relates to seeds and parts of said plants, for example fruits. The present invention further relates to methods of making and using such seeds and plants. The present invention also relates to novel genetic sequences associated with said increased resistance and to molecular markers associated with said novel genetic sequences.
Claims8
A method for identifying a cultivated watermelon plant, preferably a cultivated Citrullus lanatus subsp. lanatus plant, exhibiting resistance to FON 2 and having at least one copy of said FON 2 resistance-conferring introgressed sequence, said method comprising the step of detecting at least one of the following SNP markers:
- a) a G genotype in the heterozygous or homozygous state for SNP marker 1 at a position corresponding to position 129 in SEQ ID NO:
- 1;
- b) an A genotype in the heterozygous or homozygous state for SNP marker 2 at a position corresponding to position 120 in SEQ ID NO:
- 6;
- c) an indel genotype in the heterozygous or homozygous state for SNP marker 3 at a position corresponding to position 164 in SEQ ID NO:
- 11;
- d) an A genotype in the heterozygous or homozygous state for SNP marker 4 at a position corresponding to position 51 in SEQ ID NO:
- 16;
- e) an A genotype in the heterozygous or homozygous state for SNP marker 5 at a position corresponding to position 93 in SEQ ID NO:
- 21;
- f) a C genotype in the heterozygous or homozygous state for SNP marker 6 at a position corresponding to position 135 in SEQ ID NO:
- 26;
- g) an A genotype in the heterozygous or homozygous state for SNP marker 7 at a position corresponding to position 66 in SEQ ID NO:
- 31;
- h) an A genotype in the heterozygous or homozygous state for SNP marker 8 at a position corresponding to position 61 in SEQ ID NO:
- 36;
- i) a G genotype in the heterozygous or homozygous state for SNP marker 9 at a position corresponding to position 83 in SEQ ID NO:
- 41;
- j) an A genotype in the heterozygous or homozygous state for SNP marker 10 at a position corresponding to position 64 in SEQ ID NO:
- 46;
- k) an A genotype in the heterozygous or homozygous state for SNP marker 11 at a position corresponding to position 93 in SEQ ID NO:
- 51;
- l) an A genotype in the heterozygous or homozygous state for SNP marker 12 at a position corresponding to position 83 in SEQ ID NO:
- 56;
- m) a G genotype in the heterozygous or homozygous state for SNP marker 13 at a position corresponding to position 138 in SEQ ID NO:
- 61;
- n) a G genotype in the heterozygous or homozygous state for SNP marker 14 at a position corresponding to position 69 in SEQ ID NO:
- 66;
- and/or
- o) an A genotype in the heterozygous or homozygous state for SNP marker 15 at a position corresponding to position 51 in SEQ ID NO:
- 71;
- thereby identifying a watermelon plant exhibiting resistance to FON 2.
A method for assessing the genotype of a cultivated watermelon plant, preferably a cultivated Citrullus lanatus subsp. lanatus plant, exhibiting resistance to FON 2, said method comprising the steps of:
- a) providing a sample from said plant, and,
- b) detecting in said sample a QTL locus located on chromosome 10 and associated with said FON 2 resistance, said QTL locus being flanked by SNP markers 1 and 15, and at least one of the following SNP markers:
- i) a G genotype in the heterozygous or homozygous state for SNP marker 1 at a position corresponding to position 129 in SEQ ID NO:
- 1;
- ii) an A genotype in the heterozygous or homozygous state for SNP marker 2 at a position corresponding to position 120 in SEQ ID NO:
- 6;
- iii) an indel genotype in the heterozygous or homozygous state for SNP marker 3 at a position corresponding to position 164 in SEQ ID NO:
- 11;
- iv) an A genotype in the heterozygous or homozygous state for SNP marker 4 at a position corresponding to position 51 in SEQ ID NO:
- 16;
- v) an A genotype in the heterozygous or homozygous state for SNP marker 5 at a position corresponding to position 93 in SEQ ID NO:
- 21;
- vi) a C genotype in the heterozygous or homozygous state for SNP marker 6 at a position corresponding to position 135 in SEQ ID NO:
- 26;
- vii) an A genotype in the heterozygous or homozygous state for SNP marker 7 at a position corresponding to position 66 in SEQ ID NO:
- 31;
- viii) an A genotype in the heterozygous or homozygous state for SNP marker 8 at a position corresponding to position 61 in SEQ ID NO:
- 36;
- ix) a G genotype in the heterozygous or homozygous state for SNP marker 9 at a position corresponding to position 83 in SEQ ID NO:
- 41;
- x) an A genotype in the heterozygous or homozygous state for SNP marker 10 at a position corresponding to position 64 in SEQ ID NO:
- 46;
- xi) an A genotype in the heterozygous or homozygous state for SNP marker 11 at a position corresponding to position 93 in SEQ ID NO:
- 51;
- xii) an A genotype in the heterozygous or homozygous state for SNP marker 12 at a position corresponding to position 83 in SEQ ID NO:
- 56;
- xiii) a G genotype in the heterozygous or homozygous state for SNP marker 13 at a position corresponding to position 138 in SEQ ID NO:
- 61;
- xiv) a G genotype in the heterozygous or homozygous state for SNP marker 14 at a position corresponding to position 69 in SEQ ID NO:
- 66;
- and/or
- xv) an A genotype in the heterozygous or homozygous state for SNP marker 15 at a position corresponding to position 51 in SEQ ID NO:
- 71;
- and/or
- xvi) any other DNA marker associated with said QTL locus flanked by SNP markers 1 and 15.
A method of identifying in a cultivated watermelon plant, preferably a cultivated Citrullus lanatus subsp. lanatus plant, an introgressed sequence associated with an increased resistance to FON 2, said method comprising the step of detecting in said plant an allele of at least one DNA marker that is genetically linked to a QTL locus associated with said increased resistance to FON 2, wherein said allele maps within 10 cM, preferably within 5 cM of said QTL locus located on chromosome 10 in a genomic region flanked by SNP markers 1 and 15.
A method of identifying a cultivated watermelon plant, preferably a cultivated Citrullus lanatus subsp. lanatus plant, exhibiting increased resistance to FON 2 by identifying a QTL associated with said increased resistance to FON 2, the method comprising the steps of:
- a) detecting at least one DNA marker from a watermelon plant, which DNA marker is linked to a chromosomal interval associated with increased resistance to FON 2, wherein said chromosomal interval is flanked on each side by SNP markers having at least 80% sequence identity to SEQ ID NOs:
- 1 and 71;
- and
- b) identifying said watermelon plant comprising said at least one DNA marker.
A method of identifying a wild watermelon source of FON 2 resistance trait on chromosome 10, comprising:
- a) providing a wild watermelon accession or a plurality of wild watermelon accessions,
- b) screening said wild watermelon accession or plurality of wild watermelon accessions using a kit which detects at least one of the SNP markers listed in Table 4, and,
- c) identifying a wild watermelon accession comprising said at least one SNP marker selected in the list of Table 4.
Dependencies are inferred from the claim text.