Generating doubled haploid plants

Abstract

Provided herein are methods for generating doubled haploid plants. The methods include obtaining a haploid plant tissue, contacting the haploid plant tissue with a safener compound, contacting the haploid plant tissue with a chromosome doubling agent, and regenerating a doubled haploid plant. The safener compound (e.g., metcamifen) and the chromosome doubling agent (e.g., colchicine) are applied sequentially or simultaneously. Additionally, the methods include further contacting the haploid plant tissue with a vigor response compound (e.g., thiamethoxam) in which the vigor response compound and chromosome doubling agent are applied sequentially or simultaneously.

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