Methods for calibrating a mass spectrometry system

Abstract

The presently claimed and described technology generally relates to methods for calibrating an isoelectric focusing-mass spectrometry system. The method may include introducing an analyte mixture into a fluid channel, wherein the analyte mixture comprises at least one ampholyte and a cathodic spacer, wherein the cathodic spacer has a known mass spectral profile; applying an electric field across the fluid channel to separate the analyte mixture via isoelectric focusing; mobilizing the focused analyte misture; expelling the mobilized analyte mixture via electrospray ionization into a mass spectrometer from a single orifice; collecting a cathodic spacer mass spectrometric (MS) data comprising at least one mass spectrum from the cathodic spacer; comparing the cathodic spacer MS data with the known mass spectral profile of the cathodic spacer to generate a set of corresponding values; and adjusting one or more MS calibration parameters based on the set of corresponding values.

Dependencies are inferred from the claim text.