Representing a quantitative property of an anatomical object in a user interface

A method comprising:

• causing display, by at least one computing device and in a user interface during a medical procedure, of an image of an anatomical scene captured by an imaging device during the medical procedure;
• identifying, by the at least one computing device, a reference location in the image of the anatomical scene;
• identifying, by the at least one computing device, a plurality of additional locations in the image of the anatomical scene;
• determining, by the at least one computing device, fluorescence values representative of normalized amounts of fluorescence emitted at the plurality of additional locations relative to a normalized amount of fluorescence emitted at the reference location;
• determining, by the at least one computing device and based on the fluorescence values, one or more positions representative of a transition of the fluorescence values across a fluorescence value threshold;
• and
• causing display, by the at least one computing device and in the user interface together with the image of the anatomical scene during the medical procedure, of output data representative of the reference location, the plurality of additional locations, and the one or more transition positions.

A non-transitory computer-readable medium storing instructions that, when executed, direct a processor of a computing device to:

• cause display, in a user interface during a medical procedure, of an image of an anatomical scene captured by an imaging device during the medical procedure;
• identify a reference location in the image of the anatomical scene;
• identify a plurality of additional locations in the image of the anatomical scene;
• determine fluorescence values representative of normalized amounts of fluorescence emitted at the plurality of additional locations relative to a normalized amount of fluorescence emitted at the reference location;
• determine, based on the fluorescence values, one or more transition positions representative of a transition of the fluorescence values across a fluorescence value threshold;
• and
• cause display, in the user interface together with the image of the anatomical scene during the medical procedure, of output data representative of the reference location, the plurality of additional locations, and the one or more transition positions.