Kit and method of determining the presence or amount of creatinine
AssigneeHong Kong Centre for Cerebro-Cardiovascular Health Engineering Limited
InventorsChenyu Cui, Ting-hsuan Chen
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Abstract
A kit for determining the presence and/or amount of creatinine in a sample including: (i) an aptamer having a binding activity against creatinine; and (ii) a blocker sequence which is complementary to a portion of the aptamer for forming an aptamer-blocker duplex, wherein the blocker sequence is released from the aptamer when the aptamer binds to the creatinine in the sample. The invention also relates to a method of determining the presence and/or amount of creatinine in a sample including a step of contacting at least a portion of the sample with the aptamer-blocker duplex.
Claims18
A kit for determining the presence and/or amount of creatinine in a sample, comprising:
- (i) an aptamer having a binding activity against creatinine;
- and
- (ii) a blocker sequence which is complementary to a portion of the aptamer for forming an aptamer-blocker duplex, wherein the blocker sequence is released from the aptamer when the aptamer binds to the creatinine in the sample.
A method of determining the presence and/or amount of creatinine in a sample, comprising:
- (i) contacting at least a portion of the sample with an aptamer-blocker duplex, wherein the aptamer-blocker duplex is formed by an aptamer having a binding activity against creatinine, and a blocker sequence which is complementary to a portion of the aptamer;
- (ii) allowing the aptamer-blocker duplex to react with creatinine present in the sample under conditions for releasing the blocker sequence;
- and
- (iii) determining the presence or amount of the blocker sequence in free form in the resultant mixture, or determining the amount of aptamer-blocker duplex in the resultant mixture.
Dependencies are inferred from the claim text.