Method
AssigneePhilip Morris Products S.A.
InventorsLaura ORTEGA TORRES, Anna Grazyna GORALCZYK, Jorge André MATIAS PEREIRA, Neda HAGHAYEGH JAHROMI
Life of this patent
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Abstract
A method of measuring oxidative stress in a cell or tissue sample, the method including the steps of: (i) treating the cell or tissue sample in a medium; (ii) collecting a portion of the medium; (iii) measuring amounts of glutathione in the cell or tissue sample; and (iv) measuring amounts of reactive oxygen species (ROS) in the media portion collected in step (ii), step (iii) being carried out immediately after steps (i) and (ii), and step (iv) being carried out simultaneously with step (iii) or within 6 hours of step (iii) including assay time if the collected media portion is stored at about 0-8° C. until step (iv) is carried out.
Claims17
-15. (canceled)
A method of measuring oxidative stress in a cell or tissue sample comprising the steps of:
- (i) treating the cell or tissue sample in a medium;
- (ii) collecting a portion of the medium;
- (iii) measuring amounts of glutathione in the cell or tissue sample;
- and
- (iv) measuring amounts of reactive oxygen species (ROS) in the media portion collected in step (ii),
- wherein step (iii) is carried out immediately after steps (i) and (ii), and
- wherein step (iv) is carried out simultaneously with step (iii) or within 6 hours of step (iii) including assay time if the collected media portion is stored at about 0-8° C. until step (iv) is carried out.
A kit comprising a luminescent assay to detect and measure reduced glutathione (GSH) in biological samples like cells and tissues (GSH-Glo™ assay kit or GSH/GSSG-Glo™ kit from Promega), a reducing reagent, and instructions.
Dependencies are inferred from the claim text.