Cell labeling and annotation in micro-organospheres
AssigneeXilis, Inc.
InventorsZhaohui Wang, Xiling Shen, Eric Bankaitis, Shaun Steele
Life of this patent
Estimating the term…
Abstract
Methods and materials for labeling and annotating live cells in MicroOrganoSpheres (MOS), and methods for assessing MOS containing labeled live cells, are provided herein. The methods being applied for use in detecting treatments for diseases, particularly cancer, in mammals.
Claims29
A method comprising:
- labeling a population of cells within Micro-OrganoSpheres (MOS) with a detectable label, wherein at least a portion of the cells within the population comprise the detectable label.
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9-10. (canceled)
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A method comprising:
- providing MOS comprising a population of cells, wherein at least a portion of the cells within the population comprise a pH-sensitive detectable label,
- measuring a first level of signal from the pH-sensitive detectable label, wherein the first level of signal is directly correlated to a level of phagocytosis of the cells within the portion of cells,
- contacting the MOS with a candidate agent,
- measuring a second level of signal from the pH-sensitive detectable label, wherein the second level of signal is directly correlated to a level of phagocytosis of cells within the portion of cells after the contacting, and
- comparing the first measured level of signal to the second measured level of signal and, when the second measured level of signal is increased compared to the first measured level of signal, identifying the candidate agent as being a phagocytosis-enhancing agent for the population of cells.
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18-20. (canceled)
A method for treating a mammal, the method comprising:
- providing MOS comprising a population of cells from the mammal, wherein at least a portion of the cells within the population comprise a pH-sensitive detectable label,
- measuring a first level of signal from the pH-sensitive detectable label, wherein the first level of signal is directly correlated to a level of phagocytosis of the cells within the portion of cells,
- contacting the MOS with a candidate agent,
- measuring a second level of signal from the pH-sensitive detectable label, wherein the second level of signal is directly correlated to a level of phagocytosis of cells within the portion of cells after the contacting,
- determining that the second measured level of signal is increased compared to the first measured level of signal, and
- administering the candidate agent to the mammal.
26-28. (canceled)
A method for treating a mammal, the method comprising administering a therapeutic agent to a mammal identified as having cells that undergo an increased level of phagocytosis when contacted with the therapeutic agent, wherein the mammal was identified by:
- providing MOS comprising a population of cells from the mammal, wherein at least a portion of the cells within the population comprise a pH-sensitive detectable label,
- measuring a first level of signal from the pH-sensitive detectable label, wherein the first level of signal is directly correlated to a level of phagocytosis of the cells within the portion of cells,
- contacting the MOS with a candidate agent,
- measuring a second level of signal from the pH-sensitive detectable label, wherein the second level of signal is directly correlated to a level of phagocytosis of cells within the portion of cells after the contacting, and
- determining that the second measured level of signal is increased compared to the first measured level of signal.
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34-36. (canceled)
Dependencies are inferred from the claim text.