A wearable sensing article for body-fluid sampling, comprising:
- (a) a body-region-centered capture zone configured for placement adjacent to a target body region, including a nipple-areola complex;
- (b) a directed microfluidic inlet structure configured to concentrate micro-volume fluid into a transport path;
- (c) a conditioning layer configured to normalize a sample matrix;
- (d) a biochemical assay core configured to generate a visible or machine-readable signal;
- (e) optical calibration markers adjacent to an assay window;
- and (f) a machine-readable unique identifier, wherein the wearable sensing article is configured for single use.