Multimodal lateral flow assay system for sensitive and quantitative detection of inflammation markers

A multimodal lateral flow assay (LFA) system for non-invasively monitoring biomarkers, comprising:

• a multimodal LFA stripe, comprising:
• a sample-loading region configured to receive a biology sample comprising a biomarker;
• a conjugate region comprising triple-mode probes;
• and
• a membrane zone immobilized with a capture antibody configured to bind complexes of the triple-mode probes and the biomarker and form a test line, and a secondary antibody configured to bind the triple-mode probes and form a control line, wherein the triple-mode probes are operable to generate a colorimetric signal;
• wherein the conjugate region is disposed adjacent to the sample-loading region and the membrane zone is disposed adjacent to the conjugate region;
• a laser-emitting module configured to illuminate the membrane zone to generate a surface-enhanced Raman scattering (SERS) signal and a fluorescence signal from the triple-mode probes;
• one or more optical detection devices configured to detect and receive the SERS signal and the fluorescence signal;
• and
• a processor configured to execute a multimodal mapping for analyzing the SERS signal and the fluorescence signal to obtain a concentration of the biomarker.