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OwnerJörn GÜLDENHAUPT, Marvin MANN +6inventors
InventorsJörn GÜLDENHAUPT, Marvin MANN, Nathalie WOITZIK, Lennart Carl LANGENHOFF, Brian BUDDE, Grischa GERWERT, Carsten KÖTTING, Klaus GERWERT
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The present invention provides a molecular probe functionalized element comprising a solid base element, a first layer comprising a linker A, a second layer comprising a peptide compound, a third layer comprising a linker B, and a fourth layer comprising a molecular probe, wherein the peptide compound is covalently bound to the solid base element via linker A and the molecular probe is covalently bound to the peptide compound via linker B. The present invention also provides a process for preparing the molecular probe functionalized element and a device comprising the molecular probe functionalized element such as an optical biosensor. Further, the present invention provides a method for detecting a biomarker comprising a) bringing into contact the molecular probe functionalized element with a sample suspected to comprise a target analyte; b) detecting the biomarker based on an interaction between the molecular probe and the target analyte. The present invention also provides a use of the molecular probe functionalized element or of the device for one or more of: a companion diagnostic test; diagnosing Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Diabetes, Huntington, Prion disease, or a tumor in a patient; monitoring of therapy of patients with Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Diabetes, Huntington, Prion disease, or a tumor; and screening of drugs for the treatment of Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Diabetes, Huntington, Prion disease, or a tumor.
A molecular probe functionalized element comprising
The molecular probe functionalized element according to any one of the preceding claims, wherein linker A comprises a surface attachment group which is covalently bound to the solid base element, and wherein the surface attachment group is a silicon-containing group or a sulfur-containing group.
The molecular probe functionalized element according to any one of the preceding claims, wherein linker A comprises
The molecular probe functionalized element according to any one of the preceding claims, wherein linker A comprises
The molecular probe functionalized element according to any one of the preceding claims, wherein the first layer further comprises a matrix compound, which is covalently bound to the solid base element, wherein the matrix compound is:
The molecular probe functionalized element according to any one of the preceding claims, wherein the peptide compound of the second layer is selected from the group consisting of peptides, substituted peptides, proteins, protein fragments, and mixtures thereof, or
The molecular probe functionalized element according to any one of the preceding claims, wherein linker B comprises
The molecular probe functionalized element according to any one of the preceding claims, wherein the molecular probe is an antigen, an antibody, a fragment of an antibody, an antibody fusion protein or fusion proteins with antibody fragments, an antibody conjugate or a conjugate with an antibody fragment, a protein complex which optionally contains an antibody fragment or a fusion protein thereof, an anticalin, a nanobody, a small organic molecule, a drug, a nucleic acid, an aptamer, a lipid, a carbohydrate, a peptide, or a mixture thereof.
The molecular probe functionalized element according to any one of the preceding claims, wherein the molecular probe is capable of forming a complex with a target analyte selected from the group consisting of amyloid-beta (Aβ) peptides and isoforms thereof, alpha synuclein, tau protein, TDP-43, human islet amyloid polypeptide (hlAPP), Prion protein, and p53.
Dependencies are inferred from the claim text.