Photonic crystal biochip and protein detection method thereof

A method for preparing a photonic crystal biochip, comprising the following steps:

• 1) uniformly coating a layer of adhesive on a surface of a reaction substrate layer to obtain a substrate;
• 2) printing a photonic crystal array on the substrate using a liquid containing photonic crystal microspheres as ink to obtain a substrate with the photonic crystal array;
• 3) drying the substrate with the photonic crystal array and heat-curing;
• 4) after curing, activating the photonic crystal array, adding a capture antibody to bind with the photonic crystal array, and forming a stable capture zone;
• 5) blocking with bovine serum albumin (BSA);
• and
• 6) immobilizing a detection antibody at a periphery of the photonic crystal array to form an unstable detection zone.