Methods and compositions for functionalizing the surface of elastomeric particles
AssigneeThe Regents of the University of Colorado, a body corporate
InventorsCharles Shields, Cooper Thorne
Life of this patent
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Abstract
The invention includes systems and methods to chemically modify, and specifically functionalize the surfaces of elastomeric particles that can be used in various diagnostic and therapeutic applications. Additional embodiments of the invention include use of the functionalized elastomeric compositions to detect and isolate target analytes from heterologous fluid mixtures.
Claims25
A functionalized negative acoustic contrast particle (fNACP) comprising:
- a negative acoustic contrast particle (NACP) particle having a triethoxyvinylsilane (TEOVS) linker bound to the surface of the particle generating a surface-exposed silane or silanol group;
- an antifouling agent forming a siloxane bond with the surface-exposed group;
- and
- a recognition element operably linked to said antifouling agent.
(canceled)
13-14. (canceled)
A functionalized negative acoustic contrast particle (fNACP) comprising:
- an elastomeric particle having an identification matrix bound to its surface, the matrix comprising:
- a triethoxyvinylsilane (TEOVS) linker bound to the surface of the particle generating a surface-exposed silane or silanol group;
- a linker comprising a polyethylene glycol (PEG) molecule coupled with a first biotin molecule, wherein said PEG molecule forms a covalent siloxane bond with the surface-exposed group;
- a streptavidin (SA) molecule bound to said first biotin;
- and
- one or more biotinylated recognition elements bound to said SA, and configured to bind a target analyte.
16-26. (canceled)
A system comprising:
- a fluid sample containing a target analyte;
- a functionalized negative acoustic contrast particle (fNACP) having an identification matrix bound to its surface, the matrix comprising:
- a triethoxyvinylsilane (TEOVS) linker bound to the surface of the particle generating a surface-exposed silane or silanol group;
- a linker comprising a polyethylene glycol (PEG) molecule coupled with a first biotin molecule, wherein said PEG molecule forms a covalent siloxane bond with the surface-exposed group;
- a streptavidin (SA) molecule bound to said first biotin;
- one or more biotinylated recognition elements bound to said SA, and configured to bind the target analyte;
- and
- an acoustic separator.
30-32. (canceled)
38-66. (canceled)
Dependencies are inferred from the claim text.