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US 2026/0243756 A1Published application
3-phosphoinositide biosensors
OwnerJin Zhang, Ayse Z. Sahan +1inventors
InventorsJin Zhang, Ayse Z. Sahan, Mingyuan Chen
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Abstract
This document relates to engineered 3-phosphoinositide (3-PI) biosensor polypeptides including a lipid binding domain and a pseudoligand configured for detecting a target 3-PI in a cell via a detectable signal. In some cases, such biosensor polypeptides are useful for detecting the cellular localization of a target lipid (e.g., a target 3-PI) and/or for assessing Phosphoinositide 3-Kinase (PI3K)/Akt signaling pathway activity in a cell.
Claims45
A biosensor polypeptide comprising:
- a first linker;
- a lipid binding domain comprising the amino acid sequence set forth in SEQ ID NO:1;
- a second linker;
- a pseudoligand comprising the amino acid sequence set forth in SEQ ID NO:9;
- and
- a third linker.
3 more elements
A biosensor polypeptide comprising:
- a first linker;
- a lipid binding domain comprising the amino acid sequence set forth in SEQ ID NO:2;
- a second linker;
- a pseudoligand comprising the amino acid sequence set forth in SEQ ID NO:9;
- and
- a third linker.
3 more elements
Dependencies are inferred from the claim text.