Thin-layer chromatography identification method for characteristic components in anshen dingzhi wan

A thin-layer chromatography (TLC) identification method for characteristic components in Anshen Dingzhi Wan (ASDZW), comprising the following steps:

• S1, a spotting:
• pipetting and spotting a test solution of the ASDZW, a negative test solution of the ASDZW, a reference medicinal material solution, and a reference solution on a silica gel G thin-layer plate;
• S2, a developing:
• developing the silica gel G thin-layer plate with a developing solvent, taking out a developed silica gel G thin-layer plate after the developing, and drying the developed silica gel G thin-layer plate to obtain a dried silica gel G thin-layer plate;
• and
• S3, an inspection:
• spraying a chromogenic solution on the dried silica gel G thin-layer plate for performing a chromogenic inspection;
• wherein traditional Chinese medicinal materials for a thin-layer chromatography identification in the ASDZW comprise panax ginseng, polygala tenuifolia, poria, poria with hostwood, and acori tatarinowii rhizoma;
• wherein characteristic components in the panax ginseng comprise ginsenoside Rb1, ginsenoside Rg1, ginsenoside Re, and ginsenoside Rf;
• characteristic components in the polygala tenuifolia comprise tenuifolin;
• and characteristic components in the poria, the poria with hostwood, and the acori tatarinowii rhizoma comprise pachymic acid, dehydrotumuloic acid, and β-asarone;
• in the step S3, the inspection is conducted in a sunlight or under a 365 nm ultraviolet lamp.