Field-effect transistor biosensors integrated with porous sensing membrane

A biosensor device comprising:

• a cartridge having a chamber for receiving a fluid sample;
• a plurality of porous sensing membranes disposed in the chamber, each porous sensing membrane comprising one or more dried enzymes, enzyme-labeled antibodies, surfactants, substrates, stabilizers, buffers, or reagents disposed on or in the respective porous sensing membrane;
• and
• a plurality of working electrodes disposed in the cartridge and spaced apart from respective surfaces of the plurality of porous sensing membrane.

A system for the detection and/or analysis of a biomarker or analyte in a fluid sample comprising:

• a cartridge having a chamber for receiving the fluid sample, the cartridge comprising one or more porous sensing membrane disposed in the chamber, the one or more porous sensing membrane comprising one or more dried enzymes, enzyme-labeled antibodies, surfactants, substrates, stabilizers, buffers, or reagents disposed on or in the one or more porous sensing membrane, the cartridge further comprising one or more working electrodes disposed in the cartridge and spaced apart from respective porous sensing membrane of the one or more porous sensing membrane;
• a reference electrode configured to contact with the fluid sample in the chamber;
• one or more field-effect transistors (FETs) having a source, drain, and gate, wherein the respective gates of the one or more FETs is/are electrically coupled to the one or more working electrodes;
• and
• a semiconductor parameter analyzer or current-voltage circuitry coupled to the reference electrode and the one or more FETs.

A method for the detection and/or analysis of a biomarker or analyte in a fluid sample comprising:

• providing a cartridge having a chamber for receiving the fluid sample, the cartridge comprising one or more porous sensing membrane disposed in the chamber, the one or more porous sensing membrane comprising one or more dried enzymes, enzyme-labeled antibodies, surfactants, substrates, stabilizers, buffers, or reagents disposed on or in the one or more porous sensing membrane, the cartridge further comprising one or more working electrodes disposed in the cartridge and spaced apart from the one or more porous sensing membrane;
• providing one or more field-effect transistors (FETs) electrically coupled at respective gate(s) to the one or more working electrodes;
• loading the fluid sample containing the biomarker or analyte into the cartridge;
• measuring a change in initial threshold voltage (Vth) for the one or more FETs with a semiconductor parameter analyzer or current-voltage circuitry;
• and
• outputting a concentration of the biomarker or analyte based at least in part on the change in initial threshold voltage (Vth) for the one or more FETs.