Method of detecting target substance, reagent, and in vitro diagnostic kit
AssigneeCanon Kabushiki Kaisha
InventorsFUMIO YAMAUCHI, NORISHIGE KAKEGAWA, KENJI KITAGAWA, TSUYOSHI NOMOTO
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Abstract
Provided is a fast, simple, high-sensitivity method of detecting a target substance related to the enzyme reaction of a transglutaminase in a liquid sample, a reagent, and an in vitro diagnostic kit. Specifically, provided is a reagent for detecting a transglutaminase through use of a value related to fluorescence anisotropy, the reagent including: a luminescent particle having a first substrate of the transglutaminase; and a plurality of second substrates of the transglutaminase.
Claims19
A reagent for detecting a transglutaminase through use of a value related to fluorescence anisotropy, the reagent comprising:
- a luminescent particle having a first substrate of the transglutaminase;
- and
- a plurality of second substrates of the transglutaminase.
A reagent for detecting an activator of a transglutaminase, an activity inhibitor of the transglutaminase, and a third substrate of the transglutaminase through use of a value related to fluorescence anisotropy, the reagent comprising:
- the transglutaminase;
- a luminescent particle having a first substrate of the transglutaminase;
- and
- a plurality of second substrates of the transglutaminase.
A method of detecting a target substance related to an enzyme reaction of a transglutaminase in a liquid sample, the method comprising:
- forming, in the liquid sample containing the transglutaminase, a luminescent particle having a first substrate of the transglutaminase, and a second substrate of the transglutaminase, a crosslinked structure by a plurality of the second substrates and the first substrate that the luminescent particle has;
- and
- obtaining a value related to fluorescence anisotropy of the liquid sample after the first step.
Dependencies are inferred from the claim text.