In vitro enzymatical rna synthesis

A nucleic acid construct for generating at least one RNA product, comprising, from 5′ to 3′:

• (i) a single-stranded nucleic acid comprising at least one template part for generating the RNA product, and
• (ii) a hairpin-forming oligonucleotide which comprises successively a reverse complement of a promoter, a loop region and the promoter, said reverse complement of a promoter and said promoter being capable of forming a double-stranded oligonucleotide,
• wherein the single-stranded nucleic acid comprises at least one termination motif at the 5′ end of the template part of the single-stranded deoxyribonucleic acid, wherein said termination motif comprises at least two components selected from modified nucleotides, abasic sites, modified abasic sites, hairpin structures, protein binding sites, chemical linkers and combinations thereof.