Engineered trans-enoyl coa reductase and methods of making and using
AssigneeGenomatica, Inc.
InventorsAmit M. Shah, Harish Nagarajan, Michael Angus Noble
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Abstract
Disclosed are trans-enoyl CoA reductase (TER) enzymes and nucleic acids encoding them. In some cases, the TER enzymes are non-natural, engineered trans-enoyl CoA reductase. Also disclosed are biosynthetic methods and engineered microorganisms that enhance or improve the biosynthesis of 6-aminocaproate, hexamethylenediamine, caproic acid, caprolactone, or caprolactam. The engineered microorganisms include exogenous TER and in some cases engineered TER.
Claims16
1-72. (canceled)
A non-naturally occurring microbial organism comprising at least one engineered trans-enoyl CoA reductase (TER) enzyme comprising one or more alteration of an amino acid of SEQ ID NO:1 or SEQ ID NO:
- 24,
- wherein the one or more amino acid alterations is selected from one or more positions corresponding to residues Q11, A32, S48, Q52, S59, G97, S103, H104, V105, N106, F107, I147, S148, V149, L152, T153, L156, R200, D201, R202, V301, T302, E303, K306, N307, N308, L316, or combinations thereof of SEQ ID NO:1, or
- wherein the alterations of the amino acid is selected from one or more positions corresponding to S96, L98, M274, T275 or combinations thereof of SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.