Modification of tRNA T-Stem for Enhancing N-Methyl Amino Acid Incorporation
AssigneeThe University of Tokyo
InventorsHiroaki Suga, Takayuki Katoh, Yoshihiko Iwane
Life of this patent
Estimating the term…
Abstract
The problem to be solved by the present invention is to develop a novel peptide synthesizing method which enables the discovery of a highly N-methylated peptide having improved membrane permeability and improved stability against proteolysis and enables the simultaneous incorporation of a plurality of respectively different N-methyl amino acids. The present invention provides a tRNA having a T-stem in which N1N2GGN3 and N4CCN5U form a pair and encoding an N-methyl amino acid (N1 is G or A, one of N2 and N4 is G and the other one is C, and N3 and N4 are arbitrary amino acids forming a pair).
Claims16
A tRNA having a T-stem in which N1N2GGN3 and N4CCN5U form a pair and encoding an N-methyl amino acid (N1 is G or A;
- one of N2 and N5 is G and the other one is C;
- and N3 and N4 are any amino acids forming a pair).
Dependencies are inferred from the claim text.