Lectin-assisted isolation of mammalian cell nuclei
AssigneeTalus Bioscience, Inc.
InventorsJulia E. ROBBINS, Daniele CANZANI, Erin F. BRODERICK, Noelle C. SCHUMACKER, Lindsay K. PINO, Alexander J. FEDERATION
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Abstract
The present disclosure provides methods and compositions to handle and isolate cell nuclei and components thereof through use of lectin-coupled solid supports. The present disclosures further provide solid supports and kits and systems comprising the solid supports.
Claims47
A method of associating cell nuclei to a solid support, comprising contacting nuclei in a sample with a solid support comprising one or more lectin coupled to a surface of the solid support, wherein the one or more lectin binds to N-acetyllactosamine (LacNAc), galactose, N-acetylgalactosamine (GalNAc), and/or N-acetylglucosamine (GlcNAc), thereby associating the nuclei to the solid support.
A method of extracting nuclear proteins from cell nuclei, the method comprising:
- (a) contacting nuclei with a first buffer to extract proteins from the nuclei;
- (b) contacting the nuclei with a solid surface comprising one or more lectin coupled to a surface of the solid support, wherein the one or more lectin binds to N-acetyllactosamine (LacNAc), galactose, N-acetylgalactosamine (GalNAc), and/or N-acetylglucosamine (GlcNAc), thereby binding the nuclei to the solid support;
- and
- (c) separating the buffer and the nuclei bound to the solid support, wherein the buffer comprises the nuclear proteins.
A solid support, wherein one or more lectin that binds to N-acetyllactosamine (LacNAc), galactose, N-acetylgalactosamine (GalNAc), and/or N-acetylglucosamine (GlcNAc) is coupled to a surface of the solid support.
Dependencies are inferred from the claim text.