Mannanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsVivek SRIVASTAVA, Markus KLINGER, Rakhi SAIKA, Vijaya Shankar NATARAJ, Sohel DALAL, Jens Erik NIELSEN
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Abstract
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims13
An isolated mannanase variant of a parent mannanase, wherein said variant comprises at least two substitutions, wherein (a) said first substitution is in an amino acid position selected from the positions 260, 288, 294, or 295, and (b) said at least second substitution is selected from any other position in said variant, wherein numbering is according to SEQ ID NO:
- 2, said variant has mannanase activity and has at least 58%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO:
- 1 or the polypeptide of SEQ ID NO:
- 2.
An isolated mannanase variant of a parent mannanase, wherein the variant comprises at least two substitutions in any two or more positions corresponding to positions selected from the group of 3, 37, 47, 77, 82, 83, 93, 98, 116, 135, 136, 241, 257, and 258, wherein numbering is according to SEQ ID NO:
- 2, said variant has mannanase activity and has at least 60%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO:
- 1 or the polypeptide of SEQ ID NO:
- 2.
9-15. (canceled)
17-33. (canceled)
Dependencies are inferred from the claim text.