Adam9-targeting humanized antibody and antibody-drug conjugate comprising same and use thereof

An ADAM9-targeting antibody or an antigen-binding fragment thereof, wherein the antibody or the antigen-binding fragment thereof comprises a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises heavy chain complementarity-determining regions HCDR1, HCDR2, and HCDR3, and the light chain variable region comprises light chain complementarity-determining regions LCDR1, LCDR2, and LCDR3;

• the HCDR1 comprises the amino acid sequence shown in LYWMX1, the HCDR2 comprises the amino acid sequence shown in X2IIPIFGHTX3YX4EKFX5X6, the HCDR3 comprises the amino acid sequence shown in SEQ ID NO:
• 11, the LCDR1 comprises the amino acid sequence shown in SEQ ID NO:
• 12, the LCDR2 comprises the amino acid sequence shown in SEQ ID NO:
• 16, and the LCDR3 comprises the amino acid sequence shown in SEQ ID NO:
• 19;
• wherein X1 is N, D, H, or E;
• X2 is R or D, X3 is D or K, X4 is N or E, X5 is K or R, X6 is D or N.