Cholesterol recovery from fish oil residues
AssigneeAmerican Bioprocess Limitada
InventorsRodrigo Alejandro MANCILLA VILLALOBOS, Raúl Ignacio ARAVENA CONTRERAS
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Abstract
A method for separating and recovering cholesterol from fish oil residues, or saponified derivatives thereof, using a mixture of solvents comprising of water and alcohol. Additionally, a fatty acids-rich sub-fraction can be obtained which can be used for other applications such as biofuel production and animal feeding.
Claims27
A method for separating and recovering cholesterol from fish oil residues, or saponified derivatives thereof, comprising the stages of:
- a) saponifying a fish oil residue using an alkali in water or an alkali in a solution of water and alcohol;
- b) adding a solvent selected from the group formed by water or alcohol or a solution of water and alcohol to the mixture obtained in stage a) up to a ratio of solvent to residue from 15 to 30 w/w;
- c) maintaining the mixture obtained in stage b) at a temperature lower than the temperature of stage b) to precipitate cholesterol-rich solid;
- d) recovering the cholesterol-rich solid from the mixture after stage c);
- and
- e) washing the cholesterol-rich solid with a washing solution comprising water and alcohol.
A method for separating and recovering cholesterol from fish oil residues, or saponified derivatives thereof, comprising the stages of:
- a.1) saponifying a fish oil residue using an alkali in water or an alkali in a solution of water and alcohol;
- a.2) adding an acid to the solution obtained in a.1) up to a pH between 2 and 6;
- a.3) recovering an oily phase after adding the acid in a.2), and removing the aqueous phase and the precipitated salts obtained;
- a.4) mixing the oily phase obtained in a.3) with a solvent comprising an alkali and a solution of water and alcohol up to a ratio of solvent to residue from 15 to 30 w/w;
- a.5) maintaining the mixture obtained in stage a.4) to a temperature lower than the temperature of stage a.4) to precipitate cholesterol-rich solid;
- a.6) recovering the cholesterol-rich solid from the mixture after stage a.5);
- and
- a.7) washing the cholesterol-rich solid with a washing solution comprising water or a water and alcohol solution.
Dependencies are inferred from the claim text.