Design method and production method for nucleic acid construct and protein complex

A method for producing an agent for preventing or treating a disease, the method comprising the step of using a nucleic acid construct that induces production of an antibody against a target protein as an active ingredient, wherein the nucleic acid construct comprises:

• a polynucleotide encoding two or more T cell epitopes, wherein each of the two or more T cell epitopes being specific for an allele of an MHC class II molecule (an HLA class II molecule in the case of humans);
• and a polynucleotide encoding one or more B cell epitopes of the target protein.

A method for designing a nucleic acid construct or protein complex that induces production of an antibody against a target protein, the method comprising the step of combining two or more T cell epitopes and one or more B cell epitopes of the target protein, wherein each of the two or more T cell epitopes is specific for an allele of an MHC class II molecule (an HLA class II molecule in the case of humans).

(canceled)

A nucleic acid construct that induces production of an antibody against a target protein, the nucleic acid construct comprising:

• a polynucleotide encoding five or more T cell epitopes, wherein each of the five or more T cell epitopes being specific for an allele of an MHC class II molecule (an HLA class II molecule in the case of humans);
• and a polynucleotide encoding one or more B cell epitopes of the target protein.