Compositions derived from salvia hispanica seeds
AssigneeSignum Dermalogix, Inc.
InventorsJose Fernandez, Eddie Perez, Kristen Huber, Jason Healy, Corey Webb, Karl Rouzard, Mike Voronkov, George Nikonov
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Abstract
Methods of preparing a composition from chia seeds are provided. Compositions, including compositions prepared by the presently disclosed methods, and methods regarding selecting chia seeds and administering chia seed extracts are also disclosed.
Claims89
A method of preparing a composition from chia seeds, comprising:
- introducing a first solvent to chia seeds to form a mixture;
- agitating the mixture at a maintained temperature for a period of time above room temperature;
- filtering the chia seeds from the mixture;
- evaporating the mixture under reduced pressure to remove the first solvent and to form a crude chia seed oil;
- introducing a second solvent to the crude chia seed oil to form a solution;
- adding a decolorant to the solution;
- agitating the solution containing the decolorant for a second period of time;
- filtering the decolorant from the solution;
- and
- evaporating the second solvent from the solution under reduced pressure to prepare the composition.
The method of claim, wherein the first solvent is an ionic liquid.
The method of claim, wherein the first solvent is a polar protic solvent.
The method of claim, wherein the first solvent is a polar aprotic solvent.
The method of claim, wherein the first solvent is selected from acetonitrile, methanol, ethyl acetate, carbon disulfide, dichloromethane, trichloromethane, carbon tetrachloride, nitromethane, glyoxal, formic acid, supercritical carbon dioxide and tetramethylethane.
The method of claim, wherein the ratio of the first solvent:chia seed is about 3:1 (w/v).
The method of claim, wherein the second solvent is not toxic to humans.
The method of claim, wherein the second solvent is easily removable using vacuum evaporation at room temperature.
The method of claim, wherein the second solvent has a boiling point of less than 110° C. at 1 atm pressure.
The method of claim, wherein the second solvent is selected from methyl t-butyl ether, dichloromethane, ethyl acetate, heptane, and ethanol.
The method of claim, wherein the absorbent is activated carbon.
The method of claim, wherein the solution is agitated with the decolorant for at least 8 hours.
The method of claim, wherein the decolorant is filtered from the solution.
The method of claim, wherein the decolorant is filtered from the solution using at least two filters.
A method of preparing a composition from chia seeds, comprising:
- introducing a first polar protic solvent to chia seeds to form a mixture;
- agitating the mixture at a maintained temperature for a period of time above room temperature;
- filtering the chia seeds from the mixture;
- evaporating the mixture under reduced pressure to remove the first solvent and to form a crude chia seed oil;
- introducing a second solvent to the crude chia seed oil to form a solution;
- heating the solution to an elevated temperature for a second period of time, gradually cooling the solution to below room temperature for a third period of time, and then heating the solution to about 15-25° C. for a fourth period of time;
- and
- evaporating solvent from the solution under reduced pressure to prepare the composition.
The method of claim, further comprising adding a decolorant to the solution;
- agitating the solution containing the decolorant;
- filtering the decolorant from the solution.
The method of claim, wherein the decolorant is activated carbon.
The method of claim, wherein the first solvent and/or the second solvent is independently selected from ethanol, butanol, 2-ethylhexanol, isobutanol, isopropanol, methanol, propanol, glycerol and propylene glycol.
The method of claim, wherein the first solvent and the second solvent are ethanol.
The method of claim, wherein the ethanol is in aqueous solution and present in the solution in an amount from about 70% to about 90% (v/v).
The method of claim, wherein the ratio of the first solvent to the chia seeds is from about 8:1 to 12:1 (w/v), inclusive of any water in the first solvent.
The method of any one of the preceding claims, further comprising analyzing a sample of the composition for an amount of cis,cis,cis-9,12,15-octacedatriencoic acid (ALA) and/or an amount of cis,cis-9,12-octadecadienoic acid (LA).
The method of claim, further comprising rejecting the composition if the amount of cis,cis,cis-9,12,15-octacedatriencoic acid (ALA) is below 12 wt % and/or the amount of cis,cis-9,12-octadecadienoic acid (LA) is below 2 wt %.
A method of enriching the free acid content in chia seed oil, comprising:
- obtaining an initial chia seed oil composition;
- introducing an alcohol to the initial chia seed oil composition in the presence of a base for a period of time to form a mixture;
- introducing an acid to the mixture after the period of time;
- removing an aqueous layer from the mixture to obtain the chia seed oil with enriched free acid content.
A method of preparing a chia seed oil enriched in free acid, comprising:
- obtaining an initial chia seed oil composition and separating a portion thereof for enrichment;
- preparing an enriched free acid portion comprising:
- introducing an alcohol to the portion of the chia seed oil composition for enrichment in the presence of a base for a period of time to form a mixture;
- introducing an acid after the period of time to the mixture;
- removing an aqueous layer from the mixture to prepare the enriched free acid portion;
- adding a first amount of the enriched free acid portion to a second amount of the initial chia seed oil composition;
- washing the combined chia seed oil with an aqueous solvent and drying the combined chia seed oil.
A composition comprising an extract of chia seeds, wherein the amount of cis, cis, cis-9,12,15-octacedatriencoic acid (ALA) is at least 4 μg/mL and/or the amount of cis,cis-9,12-octadecadienoic acid (LA) is at least 0.5 μg/mL.
A composition comprising an extract of chia seeds, wherein the amount of cis, cis, cis-9,12,15-octacedatriencoic acid (ALA) is at least 8 μg/mL and the amount of cis,cis-9,12-octadecadienoic acid (LA) is less than 0.5 μg/mL.
A composition comprising an extract of chia seeds, wherein the amount of cis,cis,cis-9,12,15-octacedatriencoic acid (ALA) is at least 8 μg/mL.
A composition comprising an extract of chia seeds, wherein the amount of cis,cis-9,12-octadecadienoic acid (LA) is at least 1 μg/mL.
A method of treating a neurodegenerative disease in a subject comprising administering to the subject a composition comprising an extract of chia seeds.
A method of treating a skin disorder, medical condition or disease in a subject comprising administering to the subject a composition comprising an extract of chia seeds.
A method of treating acne in a subject comprising administering to the subject a composition comprising an extract of chia seeds.
A method of inhibiting bacterial growth on a surface comprising applying to the surface a composition comprising an extract of chia seeds.
A method of selecting a chia seed plant variety for use in preparing chia seed compositions comprising assaying said compositions for inhibition of a PP2A demethylation activity by a PP2A demethylating enzyme.
A method of selecting a chia seed plant variety for use in preparing chia seed compositions comprising assaying said compositions for cis,cis,cis-9,12,15-octacedatriencoic acid (ALA) and cis,cis-9,12-octadecadienoic acid (LA) concentrations.
A sunscreen product comprising a chia seed extract.
A sunscreen product comprising a composition prepared by the process of any one of claims to 53.
Dependencies are inferred from the claim text.